Deamidation and Isoaspartate Formation in Smeared Tau in Paired Helical Filaments
نویسندگان
چکیده
منابع مشابه
On the structure of microtubules, tau, and paired helical filaments.
Microtubules and their associated proteins form the basis of axonal transport; they are degraded during the neuronal degeneration in Alzheimer's disease. This article surveys recent results on the structure of microtubules, tau protein, and PHFs. Microtubules have been investigated by electron microscopy and image processing after labeling them with the head domain of the motor protein kinesin....
متن کاملAnthraquinones inhibit tau aggregation and dissolve Alzheimer's paired helical filaments in vitro and in cells.
The abnormal aggregation of tau protein into paired helical filaments (PHFs) is one of the hallmarks of Alzheimer's disease. Aggregation takes place in the cytoplasm and could therefore be cytotoxic for neurons. To find inhibitors of PHF aggregation we screened a library of 200,000 compounds. The hits found in the PHF inhibition assay were also tested for their ability to dissolve preformed PHF...
متن کاملAnthraquinones inhibit tau aggregation and dissolve Alzheimer paired helical filaments in vitro and in cells
The abnormal aggregation of tau protein into paired helical filaments (PHFs) is one of the hallmarks of Alzheimer’s disease. Aggregation takes place in the cytoplasm and could therefore be cytotoxic for neurons. In order to find inhibitors of PHF aggregation we screened a library of 200.000 compounds. The hits found in the PHF inhibition assay were JBC Papers in Press. Published on November 2, ...
متن کاملPurification of recombinant tau protein and preparation of Alzheimer-paired helical filaments in vitro.
The tau protein is a neuronal microtubule-associated protein. Apart of its physiological function-the binding to and stabilization of microtubules-tau is found in Alzheimer's disease brain as insoluble fibers, the so-called "paired helical filaments" (PHFs). Investigating the fundamentals of tau polymerization is indispensable for identifying inhibitory conditions or compounds preventing PHF fo...
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Recent evidence from several laboratories shows that the paired helical filaments of Alzheimer's disease brains consist mainly of the protein tau in an abnormally phosphorylated form, but the mode of assembly is not understood. Here we use EM to study several constructs derived from human brain tau and expressed in Escherichia coli. All constructs or tau isoforms are rodlike molecules with a hi...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1999
ISSN: 0021-9258
DOI: 10.1074/jbc.274.11.7368